One Arduino Mega chip based, 6 unit chemostat culturing system for microbial experiments.
all you need is Arduino Mega, mini peristaltic pump, or (whatever pump you wanna attached) Adafruit motor shield V2.0 o V2.3.
And learn how to stack your shield from here:
<@ARTDIALOG.WINNAME1573577364151 ><@ARTDIALOG.WINNAME1573577364151>https://learn.adafruit.com/adafruit-motor-shield-v2-for-arduino/stacking-shields@ARTDIALOG.WINNAME1573577364151>Then just upload the code (attached)
Use Megunolink control panel (attached in CAD file section) for controlling your machine.
Each shield control 4 pumps, two system.
3 shield control 6 systems, as you will see in the control panel there are 6 reactors.
You can easily adjust your incubation period and the feed/waste ratio.
You will need to calibrate your pumps (Time to Volume "ml").
If any questions, don't hesitate contact me through comments.
<@ARTDIALOG.WINNAME1573577364151>




<@ARTDIALOG.WINNAME1573577364151>
Best of luck.
<@ARTDIALOG.WINNAME1573577364151>
EXPANDED TECHNICAL DETAILS
Industrial Bio-Process Automation Hub
This advanced scientific instrument manages six independent chemostat bioreactors, providing a stable environment for long-term experimental evolution studies.
- Precision Fluidic Manifold: The Arduino manages twelve independent peristaltic pumps (two per unit) to maintain a constant volume and nutrient concentration. The firmware includes a "Flow Calibration" routine to ensure milliliter-precise delivery.
- MegunoLink Graphical Dashboard: Uses MegunoLink to provide a high-fidelity visual interface. Researchers can monitor Optical Density (OD), Temperature, and pH across all 6 units in real-time on their PC.
Reliability
- Watchdog Environmental Monitoring: If any unit's temperature or flow rate deviates from the setpoint for more than 5 minutes, the Arduino triggers a high-priority "System Alarm" and logs the error to an SD card.